PT  - JOURNAL ARTICLE
AU  - JING ZHAO
AU  - RONGFANG LI
AU  - ALEKSANDRA PAWLAK
AU  - MARTA HENKLEWSKA
AU  - ANGELIKA SYSAK
AU  - LIXIN WEN
AU  - JIN-E YI
AU  - BOŻENA OBMIŃSKA-MRUKOWICZ
TI  - Antitumor Activity of Betulinic Acid and Betulin in Canine Cancer Cell Lines
AID  - 10.21873/invivo.11349
DP  - 2018 Sep 01
TA  - In Vivo
PG  - 1081--1088
VI  - 32
IP  - 5
4099  - http://iv.iiarjournals.org/content/32/5/1081.short
4100  - http://iv.iiarjournals.org/content/32/5/1081.full
SO  - In Vivo2018 Sep 01; 32
AB  - Background/Aim: Betulinic acid (BA) and betulin (BT) exhibit a variety of pharmacological properties including anti-cancer, anti-inflammatory and anti-oxidant ones. Canine lymphoma and osteosarcoma have a high mortality rate and need more effective therapeutic approaches. In this study, the anti-proliferative and pro-apoptotic effects of BA and BT were investigated in canine T-cell lymphoma (CL-1), canine B-cell lymphoma (CLBL-1) and canine osteosarcoma (D-17) cell lines. Materials and Methods: The cultured cells were treated with several concentrations of BA or BT for 24, 48 and 72 h, and cell proliferation was assessed by the MTT assay. Cell apoptotic rate and cell cycle were analyzed using flow cytometry. Results: Anti-proliferative effect of BT and BA was concentration- and time-dependent. Moreover, BA and BT arrested cell cycle in S phase in CL-1 and D-17 cells, and in G0/G1 phase in CLBL-1 cells. Conclusion: Both compounds showed an antitumor activity, and the effects of BA were stronger than that of BT.