PT  - JOURNAL ARTICLE
AU  - HIROMASA FUJII
AU  - KANYA HONOKI
AU  - TOSHIFUMI TSUJIUCHI
AU  - AKIRA KIDO
AU  - KAZUHIRO YOSHITANI
AU  - YOSHINORI TAKAKURA
TI  - Growth Inhibition and Induction of Apoptosis by 2-Methoxyestradiol in Rat Osteosarcoma and Malignant Fibrous Histiocytoma Cell Lines
DP  - 2008 Jan 01
TA  - In Vivo
PG  - 21--25
VI  - 22
IP  - 1
4099  - http://iv.iiarjournals.org/content/22/1/21.short
4100  - http://iv.iiarjournals.org/content/22/1/21.full
SO  - In Vivo2008 Jan 01; 22
AB  - 2-Methoxyestradiol (2-ME) has been found to possess antitumor activity in vivo and in vitro. It has been suggested that 2-ME induces apoptosis resulting in G2/M arrest of tumor cells. In this study, the effect of 2-ME was evaluated in rat osteosarcoma and malignant fibrous histiocytoma (MFH) cell lines. 2-ME was used at final concentrations of 100 nM to 2 μM. The effect of 2-ME on cell growth was measured by the MTS assay. Induction of apoptosis and activation of caspase-3 were investigated along with apoptosis-related gene expression. The data showed that 2-ME significantly inhibited cell growth, inducing apoptosis. The activity of caspase-3 was increased at 20 h and 40 h in both cell lines. 2-ME induced p16 expression, which was possibly involved in the apoptotic process. These results suggested that the 2-ME-induced apoptosis of rat osteosarcoma and rat MFH cells was accompanied by caspase-3 activation through p16 induction. Copyright © 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved