RT Journal Article
SR Electronic
T1 TGF-beta Antisense Oligonucleotides Modulate Expression of Matrix Metalloproteinases in Isolated Fibroblasts from Radiated Skin
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 1
OP 7
VO 22
IS 1
A1 KATRIN RIEDEL
A1 T. KREMER
A1 H. RYSSEL
A1 F. RIEDEL
A1 U.R. GOESSLER
A1 E. KOELLENSPERGER
A1 G. GERMANN
A1 M. SAUERBIER
YR 2008
UL http://iv.iiarjournals.org/content/22/1/1.abstract
AB Background: Transforming growth factor-beta (TGF-beta) has been identified as an important component of wound healing. Recent developments in molecular therapy offer exciting prospects for the modulation of wound healing, specifically those targeting TGF-beta. The purpose of this study was to analyze the effect of TGF-beta targeting on the expression of matrix metalloproteinases (MMPs) in fibroblasts isolated from radiation-induced chronic dermal wounds. Materials and Methods: The expression of MMPs in tissue samples from radiation-induced chronic dermal wounds was investigated by immunohistochemistry and microarray technique. The effect of TGF-beta targeting using antisense oligonucleotides on the expression of MMPs in isolated fibroblasts was analysed by ELISA and multiplex RT-PCR. Results: Immunohistochemical investigation and microarray analysis demonstrated an increased expression of MMP protein and mRNA in tissue samples from radiation-induced chronic dermal wounds compared to normal human skin. Antisense TGF-beta oligonucleotide treatment significantly down-regulated MMP secretion in vitro. Conclusion: TGF-beta antisense oligonucleotide technology may be a potential therapeutic option for the inhibition of proteolytic tissue destruction in radiation-induced chronic wounds. Copyright © 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved