TY - JOUR T1 - TGF-beta Antisense Oligonucleotides Modulate Expression of Matrix Metalloproteinases in Isolated Fibroblasts from Radiated Skin JF - In Vivo JO - In Vivo SP - 1 LP - 7 VL - 22 IS - 1 AU - KATRIN RIEDEL AU - T. KREMER AU - H. RYSSEL AU - F. RIEDEL AU - U.R. GOESSLER AU - E. KOELLENSPERGER AU - G. GERMANN AU - M. SAUERBIER Y1 - 2008/01/01 UR - http://iv.iiarjournals.org/content/22/1/1.abstract N2 - Background: Transforming growth factor-beta (TGF-beta) has been identified as an important component of wound healing. Recent developments in molecular therapy offer exciting prospects for the modulation of wound healing, specifically those targeting TGF-beta. The purpose of this study was to analyze the effect of TGF-beta targeting on the expression of matrix metalloproteinases (MMPs) in fibroblasts isolated from radiation-induced chronic dermal wounds. Materials and Methods: The expression of MMPs in tissue samples from radiation-induced chronic dermal wounds was investigated by immunohistochemistry and microarray technique. The effect of TGF-beta targeting using antisense oligonucleotides on the expression of MMPs in isolated fibroblasts was analysed by ELISA and multiplex RT-PCR. Results: Immunohistochemical investigation and microarray analysis demonstrated an increased expression of MMP protein and mRNA in tissue samples from radiation-induced chronic dermal wounds compared to normal human skin. Antisense TGF-beta oligonucleotide treatment significantly down-regulated MMP secretion in vitro. Conclusion: TGF-beta antisense oligonucleotide technology may be a potential therapeutic option for the inhibition of proteolytic tissue destruction in radiation-induced chronic wounds. Copyright © 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -