TY - JOUR T1 - Effect of Ala16Val Genetic Polymorphism of <em>MnSOD</em> on Antioxidant Capacity and Inflammatory Response in Open Heart Surgery JF - In Vivo JO - In Vivo SP - 147 LP - 151 VL - 22 IS - 1 AU - SELIM ISBIR AU - ARZU ERGEN AU - HÜLYA YILMAZ AU - ATIKE TEKELI AU - SINAN ARSAN Y1 - 2008/01/01 UR - http://iv.iiarjournals.org/content/22/1/147.abstract N2 - Background: Ischemia-reperfusion injury and inflammation in cardiac surgical patients involves complex humoral and cellular interactions. We investigated the effect of genetic polymorphism of manganase superoxide dismutase (MnSOD) a natural antioxidant, on cytokine release and manganesuperoxide dismutase in patients undergoing coronary artery bypass grafting (CABG) with cardiopulmonary bypass (CPB). Patients and Methods: Forty-two patients undergoing elective CABG with CPB were included in the study. MnSOD polymorphism was performed by polymerase chain reaction (PCR). Levels of interleukin-6 and mangane superoxide dismutase (MnSOD) were measured by enzyme linked immunoabsorbent assay (ELISA). Results: Baseline IL-6 did not differ between patients with different MnSOD genotypes. Postoperatively IL-6 levels were significantly higher in all patients but more significantly in V(VV+AV) carriers (p=0.003). The wild-type AA genotype had the highest preoperative (p&lt;0.05) and postoperative IL-6 level. The MnSOD VV genotype was associated with significantly lower preoperative MnSOD levels compared to the AA carriers (p&lt;0.05). Conclusion: These data demonstrate that MnSOD Ala16Val polymorphism influences IL-6 production and baseline MnSOD activity, suggesting that preoperative MnSOD concentration plays a role in cytokine release. Copyright © 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -