RT Journal Article SR Electronic T1 Tenascin-C is Required for Proliferation of Astrocytes in Primary Culture JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 629 OP 633 VO 21 IS 4 A1 HIROKO IKESHIMA-KATAOKA A1 SABURO SAITO A1 SHIGEKI YUASA YR 2007 UL http://iv.iiarjournals.org/content/21/4/629.abstract AB Astrocytes in primary culture can be classified morphologically into two types: fibrous astrocytes and protoplasmic astrocytes. To examine the role of tenascin-C (TN) in an in vitro astrocyte culture, primary cultures of astrocytes prepared from the brains of wild-type and of TN-deficient embryonic mice were analyzed. In primary culture of astrocytes from TN-deficient mice fibrous astrocytes did not appear and astrocytes did not become tile-shaped when they came in contact with each other. The rate of 5-bromo-2′-deoxyuridine incorporation in a cell proliferation assay was much lower for astrocytes from TN-deficient mice than for astrocytes from wild-type mice. These results suggest that TN is an essential molecule for maintaining the proliferation and proper morphology of astrocytes in primary culture. Copyright © 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved