PT  - JOURNAL ARTICLE
AU  - IKESHIMA-KATAOKA, HIROKO
AU  - SAITO, SABURO
AU  - YUASA, SHIGEKI
TI  - Tenascin-C is Required for Proliferation of Astrocytes in Primary Culture
DP  - 2007 Jul 01
TA  - In Vivo
PG  - 629--633
VI  - 21
IP  - 4
4099  - http://iv.iiarjournals.org/content/21/4/629.short
4100  - http://iv.iiarjournals.org/content/21/4/629.full
SO  - In Vivo2007 Jul 01; 21
AB  - Astrocytes in primary culture can be classified morphologically into two types: fibrous astrocytes and protoplasmic astrocytes. To examine the role of tenascin-C (TN) in an in vitro astrocyte culture, primary cultures of astrocytes prepared from the brains of wild-type and of TN-deficient embryonic mice were analyzed. In primary culture of astrocytes from TN-deficient mice fibrous astrocytes did not appear and astrocytes did not become tile-shaped when they came in contact with each other. The rate of 5-bromo-2′-deoxyuridine incorporation in a cell proliferation assay was much lower for astrocytes from TN-deficient mice than for astrocytes from wild-type mice. These results suggest that TN is an essential molecule for maintaining the proliferation and proper morphology of astrocytes in primary culture. Copyright © 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved