%0 Journal Article %A HIROKO IKESHIMA-KATAOKA %A SABURO SAITO %A SHIGEKI YUASA %T Tenascin-C is Required for Proliferation of Astrocytes in Primary Culture %D 2007 %J In Vivo %P 629-633 %V 21 %N 4 %X Astrocytes in primary culture can be classified morphologically into two types: fibrous astrocytes and protoplasmic astrocytes. To examine the role of tenascin-C (TN) in an in vitro astrocyte culture, primary cultures of astrocytes prepared from the brains of wild-type and of TN-deficient embryonic mice were analyzed. In primary culture of astrocytes from TN-deficient mice fibrous astrocytes did not appear and astrocytes did not become tile-shaped when they came in contact with each other. The rate of 5-bromo-2′-deoxyuridine incorporation in a cell proliferation assay was much lower for astrocytes from TN-deficient mice than for astrocytes from wild-type mice. These results suggest that TN is an essential molecule for maintaining the proliferation and proper morphology of astrocytes in primary culture. Copyright © 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved %U https://iv.iiarjournals.org/content/invivo/21/4/629.full.pdf