TY - JOUR T1 - Tenascin-C is Required for Proliferation of Astrocytes in Primary Culture JF - In Vivo JO - In Vivo SP - 629 LP - 633 VL - 21 IS - 4 AU - HIROKO IKESHIMA-KATAOKA AU - SABURO SAITO AU - SHIGEKI YUASA Y1 - 2007/07/01 UR - http://iv.iiarjournals.org/content/21/4/629.abstract N2 - Astrocytes in primary culture can be classified morphologically into two types: fibrous astrocytes and protoplasmic astrocytes. To examine the role of tenascin-C (TN) in an in vitro astrocyte culture, primary cultures of astrocytes prepared from the brains of wild-type and of TN-deficient embryonic mice were analyzed. In primary culture of astrocytes from TN-deficient mice fibrous astrocytes did not appear and astrocytes did not become tile-shaped when they came in contact with each other. The rate of 5-bromo-2′-deoxyuridine incorporation in a cell proliferation assay was much lower for astrocytes from TN-deficient mice than for astrocytes from wild-type mice. These results suggest that TN is an essential molecule for maintaining the proliferation and proper morphology of astrocytes in primary culture. Copyright © 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -