TY - JOUR T1 - Biological Impact of Contact with Metals on Cells JF - In Vivo JO - In Vivo SP - 605 LP - 611 VL - 20 IS - 5 AU - TAKASHI YAMAZAKI AU - ATSUSHI YAMAZAKI AU - YASUSHI HIBINO AU - SHAHEAD ALI CHOWDHURY AU - YOSHIKO YOKOTE AU - YUMIKO KANDA AU - SHIRO KUNII AU - HIROSHI SAKAGAMI AU - HIROSHI NAKAJIMA AU - JUN SHIMADA Y1 - 2006/09/01 UR - http://iv.iiarjournals.org/content/20/5/605.abstract N2 - In order to investigate the in vivo effect of metals used in dentistry, we investigated the effect of direct contact with metal plates (20×20×0.5 mm3) made of gold (Au), silver (Ag), copper (Cu) or palladium (Pd) on human promyelocytic leukemic HL-60 cells grown in RPMI1640 medium supplemented with 10% fetal bovine serum. When 0.5 mL of cell suspension was applied to the metal plates, cells were precipitated on the surface of the metal plate within 10 min. Contact with Cu induced a rapid decline of cell viability, the smear pattern of DNA fragmentation, and only minor activation of caspase-3. These effects were accompanied by a progressive decrease in the extracellular concentration of methionine, cysteine and histidine, with a corresponding increase in the concentration of methionine sulfoxide. Electron microscopy showed that contact with Cu induced vacuolization and cytoplasmic damage, prior to nuclear damage, without affecting the cell surface microvilli or mitochondrial integrity. Contact with the other metals did not induce such changes during the 3 h incubation, nor was any hormetic response (beneficial action at lower concentration) observed in the cells with any metals. Addition of N-acetyl-L-cysteine (4-5 mM) almost completely abrogated the Cu-induced cytotoxicity, whereas sodium ascorbate (0.1-0.5 mM) and catalase (6,0001-30,000 units/mL) were ineffective. Numerous serum proteins were adsorbed to the Ag plate, while bovine serum albumin was the major protein adsorbed to other metal plates. The present study suggests that direct contact with Cu induced non-apoptotic cell death by an oxidation-involved mechanism. The present model system may be applicable to the study of the interaction between cells and dental restorative materials. Copyright © 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -