%0 Journal Article %A SANG HOON JEON %A HYUNG WOO KIM %A BIT NA KIM %A NAHYEON KANG %A CHANG DONG YEO %A CHAN KWON PARK %A YOUNG KYOON KIM %A YOON HO LEE %A TAE-JUNG KIM %A KYO YOUNG LEE %A SUG HYUNG LEE %A JONG Y. PARK %A MI SUN PARK %A HYEON WOO YIM %A SEUNG JOON KIM %T Comparison of PNA Clamping-assisted Fluorescence Melting Curve Analysis and PNA Clamping in Detecting EGFR Mutations in Matched Tumor Tissue, Cell Block, Pleural Effusion and Blood of Lung Cancer Patients With Malignant Pleural Effusion %D 2019 %R 10.21873/invivo.11516 %J In Vivo %P 595-603 %V 33 %N 2 %X Background/Aim: This study compared the efficacy of PANAMutyper™, a novel technology that integrates PNAClamp™ and PANA S-Melting™, and PNAClamp™ alone for the detection of EGFR mutations in lung cancer patients. Materials and Methods: PANAMutyper™ and PNAClamp™ were used to assess the EGFR mutation status in tissue, cell block, pleural effusion, and blood samples of 90 lung cancer patients with malignant pleural effusion. Results: PANAMutyper™ detected more EGFR mutations than PNAClamp™, especially in body fluids (pleural effusion and serum). Patients with additional EGFR mutations detected using PANAMutyper™ had a favorable response to EGFR-tyrosine kinase inhibitor (TKI) treatment. Conclusion: The diagnostic performance of PANAMutyper™ was superior to that of PNAClamp™ for the detection of EGFR mutations. It was also better at identifying lung cancer patients with malignant pleural effusion who were likely to benefit from EGFR-TKI treatment. %U https://iv.iiarjournals.org/content/invivo/33/2/595.full.pdf