TY - JOUR T1 - Overexpression of MicroRNA-1 in Prostate Cancer Cells Modulates the Blood Vessel System of an <em>In Vivo</em> Hen's Egg Test–Chorioallantoic Membrane Model JF - In Vivo JO - In Vivo SP - 41 LP - 46 DO - 10.21873/invivo.11436 VL - 33 IS - 1 AU - ARIK REUTER AU - AXEL SCKELL AU - LARS-OVE BRANDENBURG AU - MARTIN BURCHARDT AU - AXEL KRAMER AU - MATTHIAS B. STOPE Y1 - 2019/01/01 UR - http://iv.iiarjournals.org/content/33/1/41.abstract N2 - Background/Aim: In prostate cancer (PC), the formation of new blood vessels is stimulated by hypoxic conditions, androgens, and a number of molecular factors including microRNAs. MicroRNA-1 (miR-1) has been characterized in some tumor entities as anti-angiogenic, but this has not yet been investigated in PC. Materials and Methods: PC cells stably overexpressing miR-1 (LNCaP-miR-1) were incubated on an in vivo hen's egg test–chorioallantoic membrane (HET-CAM) model and compared to maternal LNCaP cells. Cell growth, blood vessel organisation, and total blood vessel area were analysed. Results: Both matrigel-embedded LNCaP and LNCaP-miR-1 cells formed compact tumor-like cell aggregates on the CAM of the HET-CAM model. Although not quantifiable, bleeding of the CAM and remodelling of the blood vessel network in the CAM indicated an influence of miR-1 on the vascular system. The statistically significant decrease in the total surface area of blood vessels in the visible CAM section to 79.4% of control cells demonstrated the antiangiogenic properties of miR-1 for the first time. Conclusion: MiR-1 had a tumor-suppressive and anti-angiogenic effect in an in vivo PC model. In the clinic, miR-1-mediated anti-angiogenesis would result in reduced tumor supply and increased hypoxic stress inside the tumor. Thus, miR-1 restoration by nucleic acid-based miR-1 mimetics would represent a promising option for future PC therapy. ER -