RT Journal Article
SR Electronic
T1 RT-PCR Expression Profiling of Matrix Metalloproteinases and their Specific Inhibitors in Cell Lines and Fresh Biopsies of Squamous Cell Carcinomas of the Head and Neck
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 943
OP 948
VO 19
IS 5
A1 A.A. DÜNNE
A1 R. MANDIC
A1 S. FALKENBERG
A1 C.V. DALCHOW
A1 A.M. SESTERHENN
A1 J.A. WERNER
YR 2005
UL http://iv.iiarjournals.org/content/19/5/943.abstract
AB The expressions of MMP2, -7, -9, -13 and TIMP1, - 2, -3 were examined in biopsies and cell lines of head and neck squamous cell carcinomas (HNSCC) to determine the association between the expression profile and TNM-staging of the primary. The expressions of MMP2, -7, -9, -13 and TIMP1, -2, -3 were analyzed in 30 HNSCC biopsies, 7 HNSCC cell lines and 1 keratinocyte cell line using RT-PCR. Negative correlation was determined between N-status and MMP13-RNA expression [Kendall-tau-b -0.404 (p=0.016), Spearman-rho -0.448 (p=0.014)], histological grading [Kendall-tau-b -0.291 (p=0.049), Spearman-rho -0,333 (p=0.048)], and MMP7 and TIMP2 expression [Kendall-tau-b -0.318 (p=0.045); Spearman-rho -0.353 (p=0.045)]. Positive correlation was determined between M-status and MMP9-RNA expression [Kendall-tau-b 0.341 (p=0.025), Spearman-rho 0.377 (p=0.024)] and MMP13 and TIMP2 expression [Kendall-tau-b 0.727 (p=0.037), Spearman-rho 0.850 (p=0.016)]. The results point to a role of the tested MMPs and TIMPs in the metastatic spread of HNSCC. Copyright © 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved