PT  - JOURNAL ARTICLE
AU  - A.A. DÜNNE
AU  - R. MANDIC
AU  - S. FALKENBERG
AU  - C.V. DALCHOW
AU  - A.M. SESTERHENN
AU  - J.A. WERNER
TI  - RT-PCR Expression Profiling of Matrix Metalloproteinases and their Specific Inhibitors in Cell Lines and Fresh Biopsies of Squamous Cell Carcinomas of the Head and Neck
DP  - 2005 Sep 01
TA  - In Vivo
PG  - 943--948
VI  - 19
IP  - 5
4099  - http://iv.iiarjournals.org/content/19/5/943.short
4100  - http://iv.iiarjournals.org/content/19/5/943.full
SO  - In Vivo2005 Sep 01; 19
AB  - The expressions of MMP2, -7, -9, -13 and TIMP1, - 2, -3 were examined in biopsies and cell lines of head and neck squamous cell carcinomas (HNSCC) to determine the association between the expression profile and TNM-staging of the primary. The expressions of MMP2, -7, -9, -13 and TIMP1, -2, -3 were analyzed in 30 HNSCC biopsies, 7 HNSCC cell lines and 1 keratinocyte cell line using RT-PCR. Negative correlation was determined between N-status and MMP13-RNA expression [Kendall-tau-b -0.404 (p=0.016), Spearman-rho -0.448 (p=0.014)], histological grading [Kendall-tau-b -0.291 (p=0.049), Spearman-rho -0,333 (p=0.048)], and MMP7 and TIMP2 expression [Kendall-tau-b -0.318 (p=0.045); Spearman-rho -0.353 (p=0.045)]. Positive correlation was determined between M-status and MMP9-RNA expression [Kendall-tau-b 0.341 (p=0.025), Spearman-rho 0.377 (p=0.024)] and MMP13 and TIMP2 expression [Kendall-tau-b 0.727 (p=0.037), Spearman-rho 0.850 (p=0.016)]. The results point to a role of the tested MMPs and TIMPs in the metastatic spread of HNSCC. Copyright © 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved