PT  - JOURNAL ARTICLE
AU  - YU-CHING LI
AU  - YU-SHENG TYAN
AU  - YIH-MIN LEE
AU  - TANG-YI TSAO
AU  - JING-YUAN CHUANG
AU  - HSIU-MAAN KUO
AU  - TE-CHUN HSIA
AU  - JEN-HUNG YANG
AU  - JING-GUNG CHUNG
TI  - N-Acetyltransferase is Involved in Baicalein-induced N-Acetylation of 2-Aminofluorene and DNA-2-Aminofluorene Adduct Formation in Human Leukemia HL-60 Cells
DP  - 2005 Mar 01
TA  - In Vivo
PG  - 399--405
VI  - 19
IP  - 2
4099  - http://iv.iiarjournals.org/content/19/2/399.short
4100  - http://iv.iiarjournals.org/content/19/2/399.full
SO  - In Vivo2005 Mar 01; 19
AB  - Many arylamine and hydrazine drugs are acetylated by cytosolic N-acetyltransferase (NAT). The human promyelocytic leukemia cell line (HL-60) has been shown to acetylate arylamine and contain NAT activity. The purpose of this study was to determine whether or not baicalein could affect N-acetylation of 2-aminofluorene (AF) in HL-60 cells. Acetylated and nonacetylated AF were determined by using high performance liquid chromatography. Baicalein displayed a dose-dependent inhibition of cytosolic and intact cells' NAT activity and reduced the number of viable cells. Time-course experiments showed that N-acetylation of AF, measured from intact HL-60 cells, was inhibited by baicalein for up to 48 h. Baicalein also decreased AF-DNA adduct formation in the examined cells. The effects of baicalein on NAT were examined by flow cytometry and NAT gene expression was examined by polymerase chain reaction. The results demonstrated that baicalein inhibited NAT1 mRNA gene expression and reduced the level of NAT in HL-60 cells. These results show that baicalein can affect the NAT activity of human leukemia cells in vitro.