RT Journal Article
SR Electronic
T1 Modulation of Branching Morphogenesis of Fetal Mouse Submandibular Gland by Sodium Ascorbate and Epigallocatechin Gallate
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 883
OP 888
VO 19
IS 5
A1 TAKAHIRO NAKA
A1 TAKAHIDE NAGAO
A1 JURI SAKAMOTO
A1 SHICHIRO MARUYAMA
A1 HIROSHI SAKAGAMI
A1 SHUJI OHKAWA
YR 2005
UL http://iv.iiarjournals.org/content/19/5/883.abstract
AB As an initial step to study the effect of antioxidants on the oral environment, we here investigated how sodium ascorbate and (-)-epigallocatechin 3-O-gallate (EGCG) affect the branching morphogenesis of the fetal mouse submandibular gland (SMG). When mouse SMG was prepared from the embryo at 13-day post prenatal stage and cultured, gradual development of branching morphogenesis was observed. Addition of sodium ascorbate affected this morphological change in a bimodal fashion. At lower concentrations of sodium ascorbate (0.25~2.27 mM), the branching morphogenesis was slightly but significantly (about 60%) enhanced, whereas at higher concentrations of sodium ascorbate (6.82~10.1 mM), the branching morphogenesis was inhibited. The addition of EGCG failed to stimulate, but inhibited the branching morphogenesis in a dose-dependent manner. These data support that the addition of a lower concentration of sodium ascorbate is essential to stimulate the growth of SMG, and that sodium ascorbate, but not all antioxidants, induces hormesis (beneficial action at lower concentration) in the present SMG system. Copyright © 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved