PT  - JOURNAL ARTICLE
AU  - KENJI DOHI
AU  - KAZUE SATOH
AU  - HIROKAZU OHTAKI
AU  - SEIJI SHIODA
AU  - YASUFUMI MIYAKE
AU  - MASATERU SHINDO
AU  - TOHRU ARUGA
TI  - Elevated Plasma Levels of Bilirubin in Patients with Neurotrauma Reflect its Pathophysiological Role in Free Radical Scavenging
DP  - 2005 Sep 01
TA  - In Vivo
PG  - 855--860
VI  - 19
IP  - 5
4099  - http://iv.iiarjournals.org/content/19/5/855.short
4100  - http://iv.iiarjournals.org/content/19/5/855.full
SO  - In Vivo2005 Sep 01; 19
AB  - Bilirubin, a powerful endogenous antioxidant, is one of the catabolites of heme oxygenases (HOs). In this study, the plasma bilirubin concentration was measured to establish bilirubin kinesis after traumatic brain injury (TBI). Furthermore, in in vitro studies, the free radical scavenging activity and antioxidant potency of bilirubin was also investigated at various concentrations, including physiological ones. Indirect plasma bilirubin was measured in 25 patients on days 1, 2, 3 and 4 after presentation with TBI. The ability of bilirubin to scavenge the hydroxyl (OH.) and 1,1-diphenyl-2-picrylhyrazyl (DPPH) radicals, and its antioxidant potency, were also analyzed using electron spin resonance (ESR) and the bioantioxidant power (BAP) methods, respectively. Plasma bilirubin levels were significantly higher on days 2, 3 and 4 than on patient admission (day 1; p<0.05). ESR and BAP results revealed that bilirubin has direct OH. and DPPH radical scavenging activities and potent antioxidant effects in vitro at physiological concentrations. These data indicate that physiological concentrations of bilirubin have antioxidant properties and that it constitutes one of the biological defense mechanisms in neurotrauma patients. Copyright © 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved