RT Journal Article
SR Electronic
T1 Establishment of an <em>In Vitro</em> Model using NR8383 Cells and <em>Mycobacterium Bovis</em> Calmette-Guérin that Mimics a Chronic Infection of <em>Mycobacterium Tuberculosis</em>
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 821
OP 830
VO 19
IS 5
A1 HINO, MAMI
A1 ODA, MUTSUMI
A1 YOSHIDA, AYA
A1 NAKATA, KAZUE
A1 KOHCHI, CHIE
A1 NISHIZAWA, TAKASHI
A1 INAGAWA, HIROYUKI
A1 HORI, HITOSHI
A1 MAKINO, KIMIKO
A1 TERADA, HIROSHI
A1 SOMA, GEN-ICHIRO
YR 2005
UL http://iv.iiarjournals.org/content/19/5/821.abstract
AB Background: Mycobacterium tuberculosis infection affects one-third of the world's population and causes the death of three million people each year. To clarify details of M. tuberculosis survival strategies, it is important to establish a suitable in vitro model that mimics a chronic infection in alveolar macrophages by M. tuberculosis. For this reason, we established a new in vitro model using a rat alveolar macrophage cell line, NR8383. Materials and Methods: Basic characteristics, including phagocytotic ability and production of nitrogen oxide and tumor necrosis factor in response to several stimuli, of NR8383 cells were compared with those of primary alveolar macrophages. The course after phagocytosis of live or killed M. bovis bacilli Calmette-Guérin (BCG) was examined over 21days using NR8383 cells as the host. Results: The characteristics that have been examined to date were nearly the same for both primary alveolar macrophage and NR8383 cells. Live BCG phagocytosed by NR8383 cells had successfully begun to grow in the cells within 7 days, while killed BCG were almost completely destroyed by 21 days. Conclusion: BCG-infected NR8383 cells are potentially a suitable in vitro model that mimics a chronic infection with M. tuberculosis. Copyright © 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved