PT  - JOURNAL ARTICLE
AU  - TADASHI MATSUMURA
AU  - NAGIO TAKIGAWA
AU  - KATSUYUKI KIURA
AU  - TAKUO SHIBAYAMA
AU  - MASAKAZU CHIKAMORI
AU  - MASAHIRO TABATA
AU  - HIROSHI UEOKA
AU  - MITSUNE TANIMOTO
TI  - Determinants of Cisplatin and Irinotecan Activities in Human Lung Adenocarcinoma Cells: Evidence of Cisplatin Accumulation and Topoisomerase I Activity
DP  - 2005 Jul 01
TA  - In Vivo
PG  - 717--721
VI  - 19
IP  - 4
4099  - http://iv.iiarjournals.org/content/19/4/717.short
4100  - http://iv.iiarjournals.org/content/19/4/717.full
SO  - In Vivo2005 Jul 01; 19
AB  - To elucidate the sensitivity of adenocarcinoma of the lung to cisplatin and irinotecan, intracellular glutathione (GSH) and glutathione S-transferase (GST)-π concentrations and topoisomerase (topo) I activity were investigated using six adenocarcinoma cell lines. The antiproliferative activity was determined by MTT assay in terms of inhibition concentration (IC50) values. The IC50 values to cisplatin were not correlated with the amounts of intracellular GSH or GST-π, but with intracellular accumulation of platinum (r=-0.91, p=0.013). IC50 values to SN-38 were correlated with topo I activity determined by relaxation assay of pBR322 (r=-0.83, p=0.040). These results suggest that platinum accumulation and topo I activity have definite impacts on the sensitivity of lung adenocarcinoma to cisplatin and irinotecan, respectively. Copyright © 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved