TY - JOUR T1 - Quest for Anti-inflammatory Substances Using IL-1β-stimulated Gingival Fibroblasts JF - In Vivo JO - In Vivo SP - 763 LP - 768 VL - 25 IS - 5 AU - MANAMI ONO AU - KAORI KANTOH AU - JUNICHI UEKI AU - AKI SHIMADA AU - HIDETSUGU WAKABAYASHI AU - TOMOHIKO MATSUTA AU - HIROSHI SAKAGAMI AU - HIDEFUMI KUMADA AU - NOBUSHIRO HAMADA AU - MADOKA KITAJIMA AU - HIROSHI OIZUMI AU - TAKAAKI OIZUMI Y1 - 2011/09/01 UR - http://iv.iiarjournals.org/content/25/5/763.abstract N2 - Background: We have previously reported that azulene-related compounds, and alkaline extract of Sasa senanensis Rehder potently inhibited nitric oxide (NO) production by lipopolysaccharide (LPS)-stimulated mouse macrophages. We investigated here whether they can inhibit pro-inflammatory cytokine production, by activated human gingival fibroblast (HGF). Materials and Methods: HGF was established from the periodontal tissues of extracted tooth. Viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Production of Prostaglandin E2 (PGE2) and cytokines was determined by enzyme immunoassay, and enzyme-linked immunosorbent assay, respectively. Results: Interleukin (IL)-1β did not inhibit, but rather slightly stimulated the growth of HGF cells. IL-1β stimulated the production of PGE2, IL-6, IL-8 and monocyte chemotactic protein-1 very potently, but not that of nitric oxide and tumor necrosis factor-α. Native LPS and synthetic lipid A from E. coli and P. gingivalis was much less stimulatory. Dexamethasone, not indomethacin, was an efficient inhibitor of IL-8 production. Among five azulene-related compounds, benzo[b]cyclohepta[e][1,4]thiazine most potently inhibited the IL-8 production by HGF cells, as well as NO production by activated RAW264.7 cells. The alkaline extract of Sasa senanensis Rehder significantly inhibited IL-8 production, without affecting the cell viability. Conclusion: The present system may be applicable for use in the search for anti-gingivitis substances. ER -