RT Journal Article
SR Electronic
T1 Interleukin 4 Increases the Antibody Response Against Rubisco in Mice
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 649
OP 652
VO 18
IS 5
A1 JÖRG A. SCHENK
A1 FRANZISKA MATYSSEK
A1 BURKHARD MICHEEL
YR 2004
UL http://iv.iiarjournals.org/content/18/5/649.abstract
AB The influence of interleukin 4 (IL-4) on antibody titer in serum and spleen culture supernatant in mice immunized with spinach (Spinacia oleracea L.) Rubisco was investigated. Therefore, we boosted one mouse additionally to the antigen with recombinant mouse IL-4. We found that the Rubisco-specific antibody titer in serum as well as in spleen cell culture supernatant was significantly enhanced in the IL-4 mouse. Most of the antibodies were of the IgG1 subclass. After hybridoma generation, Rubisco-specific antibodies were found in more than 95% of the wells tested compared to about 12% of the control mouse. Copyright © 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved