RT Journal Article
SR Electronic
T1 Analysis of the Temporal Events in Blood and Tissues Following Fecal Peritonitis in Mice
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 571
OP 576
VO 18
IS 5
A1 VANESSA V. SANTOS
A1 TANIA S. FRÖDE
A1 YARA S. MEDEIROS
YR 2004
UL http://iv.iiarjournals.org/content/18/5/571.abstract
AB Background: The kinetics of systemic responses triggered by bacterial peritonitis were analysed in the blood, bronchoalveolar lavage (BAL) and several organs. Materials and Methods: The murine model of cecal ligation and puncture was employed. The parameters were analysed at different periods of time (4 to 72 h). Results: Fecal peritonitis triggered a progressive, but not significant, decline of blood leukocytes between 4 and 6 h, becoming significant at 24 to 48 h (p<0.05). This profile was closely associated with the enhancement of leukocytes both in the abdomen (p<0.05) and in the BAL (p<0.05). A significant abdominal exudation was detected between 4 and 72 h (p<0.01), whereas maximal growth of aerobic bacteria in the blood and lungs was observed 24 and 72 h after. Maximal exudation in the studied tissues occurred at different time points (heart=24 h, spleen and kidney=48 h, liver and lung=72 h). Conclusion: Using this model, evidence of sepsis can be easily measured in different body systems. Copyright © 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved