TY - JOUR T1 - Enhancement of Wound Healing Efficacy by Chitosan-based Hydrocolloid on Sprague Dawley Rats JF - In Vivo JO - In Vivo SP - 1052 LP - 1064 DO - 10.21873/invivo.13180 VL - 37 IS - 3 AU - LINH THI THUY LE AU - NGUYEN NGAN GIANG AU - PHAM NGOC CHIEN AU - XUAN-TUNG TRINH AU - NGUYEN-VAN LONG AU - LE THI VAN ANH AU - PHAM THI NGA AU - XIN-RUI ZHANG AU - SUN-YOUNG NAM AU - CHAN-YEONG HEO Y1 - 2023/05/01 UR - http://iv.iiarjournals.org/content/37/3/1052.abstract N2 - Background/Aim: Chitosan-based functional materials have attracted considerable attention worldwide for applications in wound healing, especially in skin wound healing, due to their efficiency in hemostasis, anti-bacterial, and skin regeneration. Various chitosan-based products have been developed for skin wound healing applications, but most of these face limitations in either efficacy or cost-effectiveness. Therefore, there is a need to develop a unique material that can handle all of these concerns and be utilized for acute and chronic wounds. This study investigated mechanisms of new chitosan-based hydrocolloid patches in inflammatory reduction and skin formation by using wound-induced Sprague Dawley Rats. Materials and Methods: Our study combined a hydrocolloid patch with chitosan to achieve a practical and accessible medical patch that would enhance skin wound healing. Our chitosan-embedded patch has shown a significant influence by preventing wound expansion and inflammation increment on Sprague Dawley rat models. Results: The chitosan patch significantly increased the wound healing rate and accelerated the inflammatory stage by suppressing pro-inflammatory cytokines activity (e.g., TNF-α, IL-6, MCP-1, and IL-1β). Moreover, the product was effective in promoting skin regeneration, demonstrated by the increase in the number of fibroblasts through specific biomarkers (e.g., vimentin, α-SMA, Ki-67, collagen I, and TGF-β1). Conclusion: Our study on the chitosan-based hydrocolloid patches not only elucidated mechanisms of reducing inflammation and enhancing proliferation, but also provided a cost-effective method for skin wound dressing. ER -