RT Journal Article SR Electronic T1 Diverse Biological Activity of Odontioda Marie Noel ‘Velano’ Extracts JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 375 OP 380 VO 25 IS 3 A1 YUKO MASUDA A1 JUN-YA UEDA A1 MASAFUMI TAMURA A1 HIROSHI SAKAGAMI A1 MINEKO TOMOMURA A1 AKITO TOMOMURA A1 YOSHIAKI SHIRATAKI YR 2011 UL http://iv.iiarjournals.org/content/25/3/375.abstract AB Background: Several pharmacologically active substances have been isolated from orchid plants other than Odontioda Marie Noel ‘Velano’. Whether or not MeOH extract fractions from O. Marie Noel ‘Velano’ bulbs exert various biological activities was investigated. Materials and Methods: The MeOH extract was stepwise separated by organic solvents into n-hexane, EtOAc, n-BuOH and H2O layer fractions. Cytotoxic activity against human tumor and normal cells was determined by the MTT method. Nitric oxide (NO) was determined by the Griess method. Osteoclastogenesis was monitored by tartrate-resistant acid phosphatase (TRAP) activity. Result: The EtOAc fraction showed the highest tumor-specific cytotoxicity, followed by the n-hexane and other fractions. The EtOAc and n-BuOH fractions protected the cells from the cytotoxicity induced by UV irradiation. The EtOAc and n-hexane fractions inhibited NO production by lipopolysaccharide (LPS)-stimulated mouse macrophage-like cells. The EtOAc fraction most strongly inhibited the receptor activator for nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis, followed by the n-BuOH, n-hexane and H2O fractions. Conclusion: Most of the biological activities tested were concentrated in the EtOAc fraction, and separation from cytotoxic substances is needed to identify the active principle(s).