RT Journal Article SR Electronic T1 New Biological Activity of Rhinacanthus nasutus Extracts JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 367 OP 373 VO 25 IS 3 A1 HARUKA HORII A1 JUN-YA UEDA A1 MASAFUMI TAMURA A1 HIROSHI SAKAGAMI A1 MINEKO TOMOMURA A1 AKITO TOMOMURA A1 YOSHIAKI SHIRATAKI YR 2011 UL http://iv.iiarjournals.org/content/25/3/367.abstract AB Background: The MeOH extracts from aerial part and roots of Rhinacanthus nasutus were investigated for new biological activities. Materials and Methods: The MeOH extract of the root was stepwise separated by organic solvents into n-hexane, EtOAc, n-BuOH and H2O layer fractions. Cytotoxic activity against human tumor and normal cells was determined by the MTT method. Nitric oxide (NO) was determined by the Griess method. Osteoclastogenesis was monitored by tartrate-resistant acid phosphatase (TRAP) activity. Results: The MeOH extract of the root showed much higher tumor-specific cytotoxicity than that of the aerial part. The EtOAc fraction of the root showed the highest tumor-specific cytotoxicity, followed by the n-BuOH, n-hexane and H2O fractions. None of the four fractions protected the cells from the cytotoxicity of UV irradiation. The n-BuOH fraction not only stimulated NO production by mouse macrophage-like RAW264.7 cells, but also inhibited the lipopolysaccharide (LPS)-stimulated NO production. The EtOAc fraction inhibited the receptor activator for nuclear factor-κB ligand (RANKL)-stimulated osteoclastogenesis of the RAW264.7 cells most potently, followed by the n-hexane, n-BuOH and H2O fractions. The n-BuOH fraction slightly, but significantly stimulated osteoclastogenesis. Conclusion: Antitumor and macrophage/osteoclast-modulating substances are enriched in EtOAc and n-BuOH fractions of MeOH extract of R. nasutus roots.