TY - JOUR T1 - Carcinogen 4-Nitroquinoline Oxide (4-NQO) Induces Oncostatin-M (OSM) in Esophageal Cells JF - In Vivo JO - In Vivo SP - 506 LP - 518 DO - 10.21873/invivo.13108 VL - 37 IS - 2 AU - AMITAVA MUKHERJEE AU - MICHAEL W. EPPERLY AU - RENEE FISHER AU - DONNA SHIELDS AU - WEN HOU AU - ARJUN PENNATHUR AU - JAMES LUKETICH AU - HONG WANG AU - JOEL S. GREENBERGER Y1 - 2023/03/01 UR - http://iv.iiarjournals.org/content/37/2/506.abstract N2 - Background/Aim: The earliest cellular and molecular biologic changes in the esophagus that lead to esophageal cancer were evaluated in a mouse model. We correlated numbers of senescent cells with the levels of expression of potentially carcinogenic genes in sorted side population (SP) cells containing esophageal stem cells and non-stem cells in the non-side population cells in the 4-nitroquinolone oxide (NQO)-treated esophagus. Materials and Methods: We compared stem cells with non-stem cells from the esophagus of mice treated with the chemical carcinogen 4-NQO (100 μg/ml) in drinking water. We also compared gene expression in human esophagus samples treated with 4-NQO (100 μg/ml media) to non-treated samples. We separated and quantitated the relative levels of expression of RNA using RNAseq analysis. We identified senescent cells by luciferase imaging of p16+/LUC mice and senescent cells in excised esophagus from tdTOMp16+ mice. Results: A significant increase in the levels of RNA for oncostatin-M was found in senescent cells of the esophagus from 4-NQO-treated mice and human esophagus in vitro. Conclusion: Induction of OSM in chemically-induced esophageal cancer in mice correlates with the appearance of senescent cells. ER -