RT Journal Article SR Electronic T1 Effect of CO2 Laser Irradiation on Hormesis Induction in Human Pulp and Periodontal Ligament Fibroblasts JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 787 OP 793 VO 25 IS 5 A1 KENSUKE IWASAKA A1 ERI HEMMI A1 KAYOKO TOMITA A1 SACHIYO ISHIHARA A1 TADASHI KATAYAMA A1 HIROSHI SAKAGAMI YR 2011 UL http://iv.iiarjournals.org/content/25/5/787.abstract AB Background: We have recently reported that a low level of CO2 laser irradiation induced growth stimulation (hormesis) of both human gingival fibroblast (HGF) and oral squamous cell carcinoma cell line (HSC-2), but the extent of hormetic response was much smaller than that previously reported for toxicants and radiation in other experimental systems. Here we investigated the extent of hormetic response induced by CO2 laser irradiation in human pulp cells (HPCs) and periodontal ligament fibroblast (HPLF). Materials and Methods: HPC and HPLF cells were established from the periodontal tissues of the first premolar extracted tooth. Cells were cultured for 24, 48 or 72 hours after exposure to various irradiation powers, and the viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Results: CO2 laser irradiation induced biphasic effects on the growth of both HPC and HPLF cells. The maximum hormetic response was less than 50%. The hormetic response was found within the energy density of 7.98-79.77 J/cm2, and cytotoxicity emerged at powers over 132.96 J/cm2. Combining with our previous report, HPCs showed the highest hormetic response, followed by HPLFs and then HGFs. Both HPLFs and HGFs showed similar time-course of hormesis response, increasing response with incubation time. Conclusion: The hormetic response may be the common survival mechanism by which cells escape from radiation-induced injury. Higher hormetic response of HPCs may reflect their potential for differentiation into one of the components in dentin.