RT Journal Article
SR Electronic
T1 Changes in the Laryngeal Mucosa After Thyroid Surgery: A Rat Model
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 2224
OP 2231
DO 10.21873/invivo.12949
VO 36
IS 5
A1 SANG WOO KIM
A1 JIN YOUP KIM
A1 BO HAE KIM
A1 JOO HYUN PARK
A1 CHANG GUN CHO
A1 SEOK-WON PARK
A1 YUN-SUNG LIM
YR 2022
UL http://iv.iiarjournals.org/content/36/5/2224.abstract
AB Background/Aim: Thyroidectomy can cause various airway symptoms affecting the quality of life. We investigated the changes in extracellular matrix (ECM) composition and markers for inflammation and microcirculation of laryngeal mucosa. Materials and Methods: Sixty Sprague-Dawley rats were categorized into control and three surgical groups based on the extent of surgeries, 1) flap elevation (FE) group, 2) thyroid and trachea exposure (TE) group, and 3) thyroid isthmectomy (TI) group. We analyzed the expression of TGF-β1, VEGFR-3, CD31, and MMP- 9 in relation to the inflammatory and microcirculatory changes in the lamina propria on postoperative days (PODs) 3, 7, and 21. ECM composition of hyaluronic acid (HA) and collagen in the subglottic area (SA) was also evaluated. Results: All parameters increased in surgical groups at each postoperative phase except collagen deposition. On POD 3, TGF-β1 expression and SA increased in relation to the surgical extent and decreased over time, but more than the control in all surgical groups on POD 21. Surgical groups had more HA and less collagen composition, causing a higher HA to collagen ratio in relation to the surgical extent. VEGFR-3 and CD31 expression increased with time at all postoperative phases according to the surgical extent. Expression of MMP-9 increased in TI groups compared to TE and FE groups on POD 7 and POD 21. Conclusion: This study demonstrated that thyroid surgery exposing the thyroid and trachea induces an increase in the SA with a higher HA and lesser collagen composition. Furthermore, the markers for acute inflammation and microcirculation with tissue remodeling increased in the laryngeal mucosa.