RT Journal Article
SR Electronic
T1 Epipharyngeal Abrasive Therapy Down-regulates the Expression of Cav1.2: A Key Molecule in Influenza Virus Entry
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 2357
OP 2364
DO 10.21873/invivo.12967
VO 36
IS 5
A1 NISHI, KENSUKE
A1 YOSHIMOTO, SHOHEI
A1 NISHI, SOICHIRO
A1 NISHI, TATSURO
A1 NISHI, RYUSHIRO
A1 TSUNODA, TOSHIYUKI
A1 MORITA, HIROMITSU
A1 TANAKA, HIROAKI
A1 HOTTA, OSAMU
A1 YASUMASU, SUSUMU
A1 HIROMATSU, KENJI
A1 SHIRASAWA, SENJI
A1 NAKAGAWA, TAKASHI
A1 YAMANO, TAKAFUMI
YR 2022
UL http://iv.iiarjournals.org/content/36/5/2357.abstract
AB Background/Aim: Influenza A virus (IAV) infection causes an inflammatory response to the respiratory mucosa. The viral glycoprotein hemagglutinin (HA) binds to the sialylated voltage-dependent Ca2+ channel (Cav1.2) in ciliated epithelium. The binding of HA and sialylated Cav1.2 is considered essential to IAV infection, entry, and IAV-induced Ca2+ oscillation. The epipharynx comprises the ciliated epithelium, which is the initial target for viruses that cause upper respiratory tract infections. Previously, we showed that epipharyngeal abrasive therapy (EAT), a treatment for chronic epipharyngitis in Japan, which scratches the epipharyngeal mucosa with a cotton swab containing zinc chloride, induces squamous metaplasia. In this study, we evaluated whether squamous metaplasia by EAT affects the expression patterns of Cav1.2. Patients and Methods: The study subjects were seven patients who had not been treated with EAT and 11 patients who had. For the immunohistochemical assessment of the epipharyngeal mucosa, the staining intensity of Cav1.2 was described using the immunohistochemical score (IHC score). Results: The IHC scores for Cav1.2 in the EAT-treated group was 4.19-fold lower than those in the non-treated group (p=0.0034). Conclusion: EAT down-regulates the expression of Cav1.2, a key cell surface molecule in influenza virus entry via squamous metaplasia. Thus, EAT may be a simple method for preventing influenza infection.