RT Journal Article
SR Electronic
T1 CD3<sup>+</sup>CD56<sup>+</sup> T Lymphocytes Are Associated With ER Stress and Inflammasome Activation in Type 1 Diabetes
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 2083
OP 2091
DO 10.21873/invivo.12934
VO 36
IS 5
A1 YU-NAN HUANG
A1 WEI-DE LIN
A1 PEN-HUA SU
A1 DA-TIAN BAU
A1 FUU-JEN TSAI
A1 CHIEH-CHEN HUANG
A1 CHUNG-HSING WANG
YR 2022
UL http://iv.iiarjournals.org/content/36/5/2083.abstract
AB Background/Aim: The T cell’s flexibility of the immune system to be regulated affects the onset of type 1 diabetes (T1D). However, the mechanisms of endoplasmic reticulum (ER) stress and inflammasome activation in the circulating CD3+CD56+ T cells of patients with T1D remain unclear. This study evaluated the role of CD3+CD56+ T cells in T1D and their correlations with ER stress, inflammasome activation and disease characteristics. Materials and Methods: The frequency of circulating CD3+CD56+ T cells was determined using flow cytometry in healthy individuals and patients with T1D. Calnexin, NLR family pyrin domain containing 3 (NLRP3), ASC, caspase-1 (Casp1), cleaved caspase-3 (C-Casp3), and annexin V (AnnV) expression and propidium iodide staining in CD3+/CD56+ T cells were analyzed using flow cytometry. Results: The frequency of CD3+CD56+ T cells was reduced in patients with T1D relative to that in healthy individuals. In addition, high calnexin, NLRP3, ASC and Casp1 expression in CD3+CD56+ T cells was negatively correlated with the percentage of CD3+CD56+ T cells in patients with T1D. Conclusion: ER stress, inflammasome activation, and a lower peripheral frequency of circulating CD3+CD56+ T cells might indicate disease progression and necessitate clinical T1D immunological self-tolerance monitoring.