RT Journal Article SR Electronic T1 Beneficial Effects of Broccoli (Brassica oleracea var italica) By-products in Diet-induced Obese Mice JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 2173 OP 2185 DO 10.21873/invivo.12943 VO 36 IS 5 A1 TÂNIA MARTINS A1 RÚBEN LEITE A1 ANA FILIPA MATOS A1 JOANA SOARES A1 MARIA JOÃO PIRES A1 MARIA DE LURDES PINTO A1 MARIA JOÃO NEUPARTH A1 ANA RITA SEQUEIRA A1 LUÍS FÉLIX A1 CARLOS VENÂNCIO A1 SANDRA MARIZA MONTEIRO A1 BRUNO COLAÇO A1 IRENE GOUVINHAS A1 ANA ISABEL BARROS A1 EDUARDO ROSA A1 PAULA ALEXANDRA OLIVEIRA A1 LUÍS MIGUEL ANTUNES YR 2022 UL http://iv.iiarjournals.org/content/36/5/2173.abstract AB Background/Aim: Obesity currently affects the whole world, with greater incidence in high-income countries, with vast economic and social costs. Broccoli harvest generates many by-products equally rich in bioactive compounds with potential anti-obesity effects. This study aimed to evaluate the anti-obesity effects of broccoli by-products flour (BF) in obese mice. Materials and Methods: A commercial high-fat diet formulation (representing a Western diet) was used to induce obesity in mice. BF (0.67% or 1.34% weight/weight) was incorporated as a chemoprevention compound into a control and a hypercholesterolemic diet, at two different concentrations, and fed for 14 weeks to C57BL/6J mice. For a therapeutic approach, two groups were fed with the hypercholesterolemic diet for 10 weeks, and then fed with BF-supplemented diets in the last 4 weeks of the study. Results: BF supplementation helped to maintain a lower body weight, reduced adipose tissue accumulation, and enhanced the basal activity of superoxide dismutase and glutathione S-transferase. Although BF supplementation tended to reduce the relative liver weight increased by the Western diet, the differences were not significant. Conclusion: BF appears to have a beneficial effect in preventing weight gain and fat accumulation induced by hypercholesterolemic diets.