TY - JOUR T1 - P53 Suppressor Gene Tissue Microarray-based Protein Expression Analysis in Meningiomas JF - In Vivo JO - In Vivo SP - 2205 LP - 2210 DO - 10.21873/invivo.12946 VL - 36 IS - 5 AU - DIMITRIOS ROUKAS AU - ANASTASIOS KOUZOUPIS AU - DESPOINA SPYROPOULOU AU - GEORGE PAPANASTASIOU AU - EVANGELOS TSIAMBAS AU - GEORGE TSOUVELAS AU - EVANGELOS FALIDAS AU - VASILEIOS RAGOS AU - DIMITRIOS PESCHOS AU - LOUKAS MANAIOS AU - SPYROS KATSINIS AU - AREZINA MANOLI AU - SOTIRIOS PAPOULIAKOS AU - ANDREAS C. LAZARIS AU - NIKOLAOS KAVANTZAS Y1 - 2022/09/01 UR - http://iv.iiarjournals.org/content/36/5/2205.abstract N2 - Background/Aim: Meningiomas represent the main intracranial primary central nervous system (CNS) tumour in adults worldwide. Oncogenes’ over-activation combined with suppressor genes’ silencing affect negatively the biological behavior of these neoplasms. This study aimed to explore the impact of p53 suppressor gene expression in meningiomas’ clinic-pathological features based on a combination of sophisticated techniques. Materials and Methods: Fifty (n=50) meningiomas were included in the study, comprising a broad spectrum of histopathological subtypes. An immunohistochemistry assay was applied on tissue microarray cores followed by digital image analysis. Results: p53 protein over-expression (high staining intensity levels) was observed in 27/50 (54%) cases, whereas the rest (23/50-/46%) demonstrated moderate to low levels of the protein. p53 over-expression was statistically significantly correlated to the mitotic index of the examined cases (p-value=0.001). Interestingly, the atypical/anaplastic group of histotypes demonstrated the strongest p53 expression rates compared to the others (p-value=0.001). Conclusion: p53 overexpression is observed in a broad spectrum of meningiomas. High expression levels lead to an aggressive biological behavior of the malignancy (combined with increased mitotic rates), especially in atypical and anaplastic sub-types that also have a high recurrence rate. ER -