RT Journal Article SR Electronic T1 P53 Suppressor Gene Tissue Microarray-based Protein Expression Analysis in Meningiomas JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 2205 OP 2210 DO 10.21873/invivo.12946 VO 36 IS 5 A1 DIMITRIOS ROUKAS A1 ANASTASIOS KOUZOUPIS A1 DESPOINA SPYROPOULOU A1 GEORGE PAPANASTASIOU A1 EVANGELOS TSIAMBAS A1 GEORGE TSOUVELAS A1 EVANGELOS FALIDAS A1 VASILEIOS RAGOS A1 DIMITRIOS PESCHOS A1 LOUKAS MANAIOS A1 SPYROS KATSINIS A1 AREZINA MANOLI A1 SOTIRIOS PAPOULIAKOS A1 ANDREAS C. LAZARIS A1 NIKOLAOS KAVANTZAS YR 2022 UL http://iv.iiarjournals.org/content/36/5/2205.abstract AB Background/Aim: Meningiomas represent the main intracranial primary central nervous system (CNS) tumour in adults worldwide. Oncogenes’ over-activation combined with suppressor genes’ silencing affect negatively the biological behavior of these neoplasms. This study aimed to explore the impact of p53 suppressor gene expression in meningiomas’ clinic-pathological features based on a combination of sophisticated techniques. Materials and Methods: Fifty (n=50) meningiomas were included in the study, comprising a broad spectrum of histopathological subtypes. An immunohistochemistry assay was applied on tissue microarray cores followed by digital image analysis. Results: p53 protein over-expression (high staining intensity levels) was observed in 27/50 (54%) cases, whereas the rest (23/50-/46%) demonstrated moderate to low levels of the protein. p53 over-expression was statistically significantly correlated to the mitotic index of the examined cases (p-value=0.001). Interestingly, the atypical/anaplastic group of histotypes demonstrated the strongest p53 expression rates compared to the others (p-value=0.001). Conclusion: p53 overexpression is observed in a broad spectrum of meningiomas. High expression levels lead to an aggressive biological behavior of the malignancy (combined with increased mitotic rates), especially in atypical and anaplastic sub-types that also have a high recurrence rate.