PT - JOURNAL ARTICLE AU - KAZUYUKI HAMADA AU - YUTARO KUBOTA AU - YUSUKE AOKI AU - NORIHIKO SUGISAWA AU - JUN YAMAMOTO AU - YOSHIHIKO TASHIRO AU - MICHAEL BOUVET AU - TAKUYA TSUNODA AU - ROBERT M. HOFFMAN TI - Obesity Strongly Promotes Growth of Mouse MC38 Colon Cancer in an Orthotopic-syngeneic C57BL/6 Mouse Model AID - 10.21873/invivo.12875 DP - 2022 Jul 01 TA - In Vivo PG - 1643--1646 VI - 36 IP - 4 4099 - http://iv.iiarjournals.org/content/36/4/1643.short 4100 - http://iv.iiarjournals.org/content/36/4/1643.full SO - In Vivo2022 Jul 01; 36 AB - Background/Aim: Obesity is a major risk factor for colorectal cancer. The MC38 mouse colon-cancer cell line is a versatile syngeneic model of colon cancer in C57BL/6 mice. In the present study, the influence of a high-fat diet (HFD) on the growth of the MC38 mouse colon-cancer cell line was examined in an orthotopic-transplantation syngeneic model in C57BL/6 mice. Materials and Methods: Five 6-week-old C57BL/6 male mice were fed a control diet (CD, 6.5% fat) or HFD (34.3% fat) for eight weeks. Then, a 2 mm3 fragment of a subcutaneous MC38 tumor was attached to the surface of the cecum of C57BL/6 mice with a single stitch using a 7-0 suture to establish an orthotopic-transplantation model. Each group continued their initial diet for 17 days. Results: The HFD group had more than twice the tumor volume and tumor weight than the CD group (p=0.021 and p=0.014, respectively). Conclusion: HFD-induced obesity strongly increased MC38 colon-cancer progression in a C57BL/6 orthotopic-transplantation mouse model. The present study emphasizes the detrimental effect of obesity on coloncancer progression.