RT Journal Article
SR Electronic
T1 Contact Co-culture of Osteoblasts and Sympathetic Neuronal Cells Promotes Differentiation of Both Cell Types
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 1608
OP 1614
DO 10.21873/invivo.12871
VO 36
IS 4
A1 MASAHIKO OKUBO
A1 KO ITO
A1 FUMIE YAMAZAKI
A1 YOSUKE MIZUNO
A1 YUTA ISOZAKI
A1 SEIJI ASODA
A1 MICHIHIKO USUI
A1 TSUYOSHI SATO
YR 2022
UL http://iv.iiarjournals.org/content/36/4/1608.abstract
AB Background/Aim: Bone and nerve reconstruction is crucial for treating various diseases of the oral and maxillofacial region. However, the relationship between bone and nervous system has not yet been fully elucidated. Therefore, we aimed to examine the interaction between osteoblasts and neuronal cells in contact co-culture. Material and Methods: Osteoblasts and sympathetic neuronal cells were grown in contact co-culture. Microscopic observation, a mineralization assay, immunofluorescence staining, and DNA microarray analysis were performed. Results: Microscopic observation revealed morphological changes in the osteoblasts that were cocultured with sympathetic neuronal cells. Contact co-culture enhanced osteoblast calcification and upregulated a neuronal marker. Not only osteoblast differentiation signals, but also neuronal signals were increased in murine osteoblasts that were co-cultured with rat sympathetic neuronal cells. We also found that not only rat neuron differentiation signals, but also osteoblast differentiation signals were increased in rat sympathetic neuronal cells that were co-cultured with murine osteoblasts. Conclusion: In the contact co-culture with osteoblasts and sympathetic neuronal cells, the sympathetic neuronal cells promoted osteoblast differentiation, and the osteoblasts promoted neuron differentiation.