RT Journal Article
SR Electronic
T1 <em>Agrimonia eupatoria</em> L. Aqueous Extract Improves Skin Wound Healing: An <em>In Vitro</em> Study in Fibroblasts and Keratinocytes and <em>In Vivo</em> Study in Rats
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 1236
OP 1244
DO 10.21873/invivo.12822
VO 36
IS 3
A1 TOMÁŠ VASILENKO
A1 IVAN KOVÁČ
A1 MARTIN SLEZÁK
A1 JÁN ĎURKÁČ
A1 VLASTA PERŽEL’OVÁ
A1 MATÚŠ ČOMA
A1 MIRIAM KAŇUCHOVÁ
A1 LUKÁŠ URBAN
A1 PAVOL SZABO
A1 BARBORA DVOŘÁNKOVÁ
A1 ANDREJ VRZGULA
A1 ROBERT ZAJÍČEK
A1 KAREL SMETANA, JR.
A1 PETER GÁL
YR 2022
UL http://iv.iiarjournals.org/content/36/3/1236.abstract
AB Background/Aim: We have previously shown that the water extract of Agrimonia eupatoria L. (AE) is a valuable source of polyphenols with excellent antioxidant properties and has clinical potential for the prevention and/or adjuvant therapy of cardiovascular complications associated with diabetes. Inspired by our previously published data, in the present study we examined whether AE improves skin wound healing in a series of in vitro and in vivo experiments. Materials and Methods: In detail, we investigated the ability of the AE extract to induce fibroblast to myofibroblast conversion, extracellular matrix (ECM) deposition, and keratinocyte proliferation/differentiation, in vitro. In parallel, in an animal model, we measured wound tensile strength (TS) and assessed the progression of open wounds using basic histology and immunofluorescence. Results: The AE extract induced the myofibroblast-like phenotype and enhanced ECM deposition, both in vitro and in vivo. Furthermore, the wound TS of skin incisions and the contraction rates of open excisions were significantly increased in the AE-treated group. Conclusion: The present data show that AE water extract significantly improves the healing of open and sutured skin wounds. Therefore, our data warrant further testing in animal models that are physiologically and evolutionarily closer to humans.