TY - JOUR T1 - Antibacterial and Resistance Modifying Activities of <em>Nigella sativa</em> Essential Oil and its Active Compounds Against <em>Listeria monocytogenes</em> JF - In Vivo JO - In Vivo SP - 737 LP - 743 DO - 10.21873/invivo.11302 VL - 32 IS - 4 AU - AHMAD MOUWAKEH AU - ÁGNES TELBISZ AU - GABRIELLA SPENGLER AU - CSILLA MOHÁCSI-FARKAS AU - GABRIELLA KISKÓ Y1 - 2018/07/01 UR - http://iv.iiarjournals.org/content/32/4/737.abstract N2 - Background/Aim: N. sativa essential oil (EO) and its compounds (thymoquinone, carvacrol and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol and p-cymene against Listeria monocytogenes. Materials and Methods: N. sativa EO, thymoquinone, carvacrol and p-cymene was assessed for its antimicrobial activity, modulation of antimicrobial resistance, inhibition of antimicrobial efflux and membrane integrity by broth microdilution, ethidium bromide accumulation and LIVE/DEAD BacLight™ assays. Results: L. monocytogenes showed substantial susceptibility toward N. sativa EO, thymoquinone, and carvacrol. A significant reduction in MIC's of EtBr and ciprofloxacin was noticed when tested in combination with N. sativa EO, thymoquinone, carvacrol and reserpine. In the presence of each compound the membrane integrity was disintegrated, and the EtBr accumulation increased which was comparable to positive control reserpine. Conclusion: N. sativa EO might have a potential for controlling the antibiotic resistance in Listeria. ER -