RT Journal Article
SR Electronic
T1 Synergistic Effects of BTK Inhibitor HM71224 and Methotrexate in a Collagen-induced Arthritis Rat Model
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 3245
OP 3251
DO 10.21873/invivo.12619
VO 35
IS 6
A1 KYUNG JIN CHOI
A1 YU-YON KIM
A1 SUN YOUNG JANG
A1 YOUNG GIL AHN
A1 KWEE HYUN SUH
A1 YOUNG HOON KIM
A1 HYUNG SIK KIM
YR 2021
UL http://iv.iiarjournals.org/content/35/6/3245.abstract
AB Background/Aim: Using a rat model of collagen-induced arthritis (CIA), we evaluated the therapeutic effects of HM71224 (BTKi), as well as the drug-drug interactions in combined therapy with methotrexate (MTX) based on both drugs’ pharmacological role in immune regulation and antiinflammation. Materials and Methods: Arthritis in rats was induced using type II collagen and incomplete Freund’s adjuvant. The therapeutic effects of HM71224 (alone or in combination with MTX) were evaluated by arthritis score, paw volume, body weight, and histopathological examination (H&E and Safranin-O staining). The drug-drug interactions between HM71224 and MTX were investigated by measuring plasma, liver enzyme and creatinine levels and blood cell counts. Results: HM71224 reduced the clinical signs of arthritis, paw volume, and body weight loss in CIA rats. ED50 and ED90 were 1.0 and 2.5 mg/kg, respectively. HM71224 combined with MTX decreased the arthritis score, bone erosion, synovitis, and cartilage degradation without apparent interaction. Conclusion: The combination of HM71224 and MTX improved the therapeutic effect with no drug-drug interactions in RA.