RT Journal Article
SR Electronic
T1 Curcumin Derivative MTH-3 Regulates Palmitate-induced Insulin Resistance in Mouse Myoblast C<sub>2</sub>C<sub>12</sub> Cells
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 3181
OP 3191
DO 10.21873/invivo.12613
VO 35
IS 6
A1 YU-JEN CHIU
A1 YU-HSIANG LO
A1 JAI-SING YANG
A1 SHENG-CHU KUO
A1 SHIH-CHANG TSAI
YR 2021
UL http://iv.iiarjournals.org/content/35/6/3181.abstract
AB Background/Aim: At present, there are no effective drugs for the treatment of insulin resistance. MTH-3, a curcumin derivative, exerts potent anti-cancer effects. The aim of the present study was to explore whether MTH-3 is capable of regulating palmitic acid (PA)-induced insulin resistance in C2C12 cells. Materials and Methods: Cell viability was examined using the MTT assay. C2C12 cells were treated with PA and evaluated for the production of oil droplets using an Oil Red O assay. Glucose uptake was analysed by the 2-NBDG assay. Results: Treatment of cells with up to 500 μM PA for 24 h or with 5 or 10 μM MTH-3 had no effect on cell viability. PA induced production of oil droplets in C2C12 cells. After adding MTH-3, the quantity of oil droplets decreased significantly and glucose uptake recovered. Conclusion: MTH-3 may become an efficient drug for the treatment of insulin resistance and associated diseases.