PT - JOURNAL ARTICLE AU - YU-JEN CHIU AU - YU-HSIANG LO AU - JAI-SING YANG AU - SHENG-CHU KUO AU - SHIH-CHANG TSAI TI - Curcumin Derivative MTH-3 Regulates Palmitate-induced Insulin Resistance in Mouse Myoblast C<sub>2</sub>C<sub>12</sub> Cells AID - 10.21873/invivo.12613 DP - 2021 Nov 01 TA - In Vivo PG - 3181--3191 VI - 35 IP - 6 4099 - http://iv.iiarjournals.org/content/35/6/3181.short 4100 - http://iv.iiarjournals.org/content/35/6/3181.full SO - In Vivo2021 Nov 01; 35 AB - Background/Aim: At present, there are no effective drugs for the treatment of insulin resistance. MTH-3, a curcumin derivative, exerts potent anti-cancer effects. The aim of the present study was to explore whether MTH-3 is capable of regulating palmitic acid (PA)-induced insulin resistance in C2C12 cells. Materials and Methods: Cell viability was examined using the MTT assay. C2C12 cells were treated with PA and evaluated for the production of oil droplets using an Oil Red O assay. Glucose uptake was analysed by the 2-NBDG assay. Results: Treatment of cells with up to 500 μM PA for 24 h or with 5 or 10 μM MTH-3 had no effect on cell viability. PA induced production of oil droplets in C2C12 cells. After adding MTH-3, the quantity of oil droplets decreased significantly and glucose uptake recovered. Conclusion: MTH-3 may become an efficient drug for the treatment of insulin resistance and associated diseases.