@article {CHIU3181, author = {YU-JEN CHIU and YU-HSIANG LO and JAI-SING YANG and SHENG-CHU KUO and SHIH-CHANG TSAI}, title = {Curcumin Derivative MTH-3 Regulates Palmitate-induced Insulin Resistance in Mouse Myoblast C2C12 Cells}, volume = {35}, number = {6}, pages = {3181--3191}, year = {2021}, doi = {10.21873/invivo.12613}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: At present, there are no effective drugs for the treatment of insulin resistance. MTH-3, a curcumin derivative, exerts potent anti-cancer effects. The aim of the present study was to explore whether MTH-3 is capable of regulating palmitic acid (PA)-induced insulin resistance in C2C12 cells. Materials and Methods: Cell viability was examined using the MTT assay. C2C12 cells were treated with PA and evaluated for the production of oil droplets using an Oil Red O assay. Glucose uptake was analysed by the 2-NBDG assay. Results: Treatment of cells with up to 500 μM PA for 24 h or with 5 or 10 μM MTH-3 had no effect on cell viability. PA induced production of oil droplets in C2C12 cells. After adding MTH-3, the quantity of oil droplets decreased significantly and glucose uptake recovered. Conclusion: MTH-3 may become an efficient drug for the treatment of insulin resistance and associated diseases.}, issn = {0258-851X}, URL = {https://iv.iiarjournals.org/content/35/6/3181}, eprint = {https://iv.iiarjournals.org/content/35/6/3181.full.pdf}, journal = {In Vivo} }