RT Journal Article
SR Electronic
T1 Presence of HPV 16 and HPV 18 in Spermatozoa and Embryos of Mice
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 3203
OP 3209
DO 10.21873/invivo.12615
VO 35
IS 6
A1 EIRINI MASTORA
A1 CHRYSOULA KITSOU
A1 THEOCHARIS EVANGELOU
A1 ATHANASIOS ZIKOPOULOS
A1 NEKTARIA ZAGORIANAKOU
A1 IOANNIS GEORGIOU
YR 2021
UL http://iv.iiarjournals.org/content/35/6/3203.abstract
AB Background/Aim: Sperm cells are competent to integrate exogenous DNA into their genome. We sought to clarify Human Pappiloma Virus (HPV) internalization in spermatozoa and early preimplantation embryos. Materials and Methods: Sperm was incubated with plasmid vectors containing the complete genome of human HPV 16 and HPV 18 tagged with the green fluorescent protein (GFP) gene, to investigate HPV 16 and HPV 18 integration in mouse spermatozoa. Oocytes were in vitro fertilized with preincubated spermatozoa to investigate HPV 16 and HPV 18 potential transfer to mouse embryos. Results: Spermatozoa were able to internalize constructs of cloned high-risk HPV either as integrated or as episomal DNA. Constructs of cloned HPV can also be transferred to mouse embryos, through in vitro fertilization of the oocytes by mouse spermatozoa. Conclusion: Viral DNA transmission to the early mouse embryo via sperm, highlights the effect of HPV in reproductive cells and preimplantation development.