%0 Journal Article %A AFRODITE SIALAKOUMA %A IOANNIS KARAKASILIOTIS %A VAIA NTALA %A NIKOLAOS NIKOLETTOS %A BYRON ASIMAKOPOULOS %T Embryonic Cell-free DNA in Spent Culture Medium: A Non-invasive Tool for Aneuploidy Screening of the Corresponding Embryos %D 2021 %R 10.21873/invivo.12645 %J In Vivo %P 3449-3457 %V 35 %N 6 %X Background/Aim: Preimplantation genetic testing (PGT) for chromosomal screening, based on embryo biopsy, has significant limitations. Cell-free DNA (cf-DNA) has been detected in spent culture medium (SCM), opening new horizons for the development of non-invasive PGT (ni-PGT). In this study, we evaluated the diagnostic performance of ni-PGT for aneuploidy (niPGT-A), comparing the results of trophectoderm biopsies (TE) and respective SCM from individually cultured embryos via Next Generation Sequencing (NGS). Materials and Methods: Forty fresh embryos were analyzed. TE and SCM from blastocysts were collected and analyzed. Results: We detected cfDNA in 100% of samples tested. The overall concordance rate between the ni-PGT-A and PGT-A was 27/33 (81.8%). The full concordance rate was 21/33 (63.6%). The aneuploidy agreement was 91.66%, and the euploidy agreement was 76.19%. Conclusion: We found a good accordance between TE and SCM analysis, suggesting that niPGT-A could be a reliable alternative for chromosomal abnormalities assessment of in vitro cultured embryos. %U https://iv.iiarjournals.org/content/invivo/35/6/3449.full.pdf