PT  - JOURNAL ARTICLE
AU  - NATTHA SUWANPRAKORN
AU  - PITHI CHANVORACHOTE
AU  - THITIPORN TONGYEN
AU  - BOONCHOO SRITULARAK
AU  - PICHIT SUVANPRAKORN
TI  - Scoparone Induces Expression of Pluripotency Transcription Factors SOX2 and NANOG in Dermal Papilla Cells
AID  - 10.21873/invivo.12541
DP  - 2021 Sep 01
TA  - In Vivo
PG  - 2589--2597
VI  - 35
IP  - 5
4099  - http://iv.iiarjournals.org/content/35/5/2589.short
4100  - http://iv.iiarjournals.org/content/35/5/2589.full
SO  - In Vivo2021 Sep 01; 35
AB  - Background/aim: Dermal papilla cells (DPCs) regulate hair follicle development. We aimed to investigate the effect of scoparone from Dendrobium densiflorum on DPCs in the induction of stem cell properties and pluripotency-related proteins. Materials and methods: DPC viability was evaluated by the MTT assay. Apoptosis or necrosis of DPCs was determined by Hoecsht33342/PI nuclear staining analysis. Expression of OCT4, NANOG and SOX2 genes was determined using Real-Time Polymerase Chain Reaction (PCR). Immunocytochemistry and western blot analysis were performed to determine pluripotency related proteins. Results: Scoparone increased the expression of pluripotency related transcription factors SOX2 and NANOG, while it had minimal effects on OCT4 levels. Scoparone exerted its stemness-enhancing activity through the up-regulation of Akt-dependent inhibition of GSK3β, resulting in increased cellular levels of β-catenin. Conclusion: Our results show a potential novel activity and mechanism of action of scoparone on human DPCs that could facilitate the development of hair enrichment approaches.