RT Journal Article SR Electronic T1 Inhibition of Toll Like Signaling Pathway Is Associated With Genomic Instability in Rat Liver Exposed to Crack Cocaine JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 2641 OP 2646 DO 10.21873/invivo.12546 VO 35 IS 5 A1 DANIEL VITOR DE SOUZA A1 DOS ANJOS BARBARA ROSARIO A1 LAIS VALES MENNITTI A1 INGRA TAIS MALACARNE A1 LUCIANA PELLEGRINI PISANI A1 REGINA CLAUDIA BARBOSA DA SILVA A1 MILENA DE BARROS VIANA A1 DANIEL ARAKI RIBEIRO YR 2021 UL http://iv.iiarjournals.org/content/35/5/2641.abstract AB Background/Aim: The aim of the present study was to investigate the biological effects of subacute crack cocaine exposure in rat liver. Material and Methods: A total of 32 rats were distributed into four groups (n=8): Experimental group 1 (G1) and Experimental group 2 (G2): rats received 18 mg/kg of body weight (b.w) of crack cocaine for 5 days, once a day, group G2 remained 72 h without exposure after the experimental period (5 days)(abstinence); Experimental group 3 (G3): rats received 36 mg/kg of body weight (b.w) of crack cocaine for 5 days, once a day; Control Group (CTRL): rats received only the vehicle (DMSO) administered by the intraperitoneal (i.p) route for 5 days, once a day. Results: All groups exposed to crack cocaine had an increase in the number of micronucleated hepatocytes and binucleated cells only in the highest tested dose (36 mg/kg). Karyolysis had an increase in the 18 mg/kg dose, in the abstinence group (G2), and 36 mg/kg group (G3); whereas pyknotic nuclei had an increase in the G2 group. The group exposed to 18 mg/kg of crack cocaine also showed high 8 OHdG expression. The p-NF-κB p65 protein decreased in the groups exposed to crack cocaine at doses of 18 and 36 mg/kg, as well as in the abstinence group. MyD88 was also found decreased in the group exposed to crack cocaine at 18 mg/kg. Conclusion: Crack cocaine inhibited toll like signaling pathway whilst being associated with genomic instability in rat liver cells.