PT - JOURNAL ARTICLE AU - DANIEL VITOR DE SOUZA AU - DOS ANJOS BARBARA ROSARIO AU - LAIS VALES MENNITTI AU - INGRA TAIS MALACARNE AU - LUCIANA PELLEGRINI PISANI AU - REGINA CLAUDIA BARBOSA DA SILVA AU - MILENA DE BARROS VIANA AU - DANIEL ARAKI RIBEIRO TI - Inhibition of Toll Like Signaling Pathway Is Associated With Genomic Instability in Rat Liver Exposed to Crack Cocaine AID - 10.21873/invivo.12546 DP - 2021 Sep 01 TA - In Vivo PG - 2641--2646 VI - 35 IP - 5 4099 - http://iv.iiarjournals.org/content/35/5/2641.short 4100 - http://iv.iiarjournals.org/content/35/5/2641.full SO - In Vivo2021 Sep 01; 35 AB - Background/Aim: The aim of the present study was to investigate the biological effects of subacute crack cocaine exposure in rat liver. Material and Methods: A total of 32 rats were distributed into four groups (n=8): Experimental group 1 (G1) and Experimental group 2 (G2): rats received 18 mg/kg of body weight (b.w) of crack cocaine for 5 days, once a day, group G2 remained 72 h without exposure after the experimental period (5 days)(abstinence); Experimental group 3 (G3): rats received 36 mg/kg of body weight (b.w) of crack cocaine for 5 days, once a day; Control Group (CTRL): rats received only the vehicle (DMSO) administered by the intraperitoneal (i.p) route for 5 days, once a day. Results: All groups exposed to crack cocaine had an increase in the number of micronucleated hepatocytes and binucleated cells only in the highest tested dose (36 mg/kg). Karyolysis had an increase in the 18 mg/kg dose, in the abstinence group (G2), and 36 mg/kg group (G3); whereas pyknotic nuclei had an increase in the G2 group. The group exposed to 18 mg/kg of crack cocaine also showed high 8 OHdG expression. The p-NF-κB p65 protein decreased in the groups exposed to crack cocaine at doses of 18 and 36 mg/kg, as well as in the abstinence group. MyD88 was also found decreased in the group exposed to crack cocaine at 18 mg/kg. Conclusion: Crack cocaine inhibited toll like signaling pathway whilst being associated with genomic instability in rat liver cells.