RT Journal Article
SR Electronic
T1 The Effects of Trypsin Inhibitor on Insulin Secretion Using Rat Pancreas in an Organ Bath
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 2551
OP 2558
DO 10.21873/invivo.12537
VO 35
IS 5
A1 MORITA, ASUKA
A1 OUCHI, MOTOSHI
A1 SATOH, KEITARO
A1 KOBAYASHI, SHUNSUKE
A1 TERADA, MISAO
A1 WAKASHIN, HIDEFUMI
A1 KON, HIROE
A1 HAYASHI, KEITARO
A1 ANZAI, NAOHIKO
A1 SHIMIZU, AKIRA
A1 SUGIHARA, HITOSHI
A1 OBA, KENZO
A1 FUJITA, TOMOE
YR 2021
UL http://iv.iiarjournals.org/content/35/5/2551.abstract
AB Background/Aim: We developed an experimental method to reproduce insulin secretion from isolated rat pancreas preparations using an organ bath system. However, secretion of trypsin, another pancreatic enzyme, interferes with insulin production in such systems. We aimed to ascertain the minimum trypsin inhibitor (TI), dose for obtaining a sustained, stable rate of insulin secretion. Materials and Methods: The action of TI (1-10 μg/ml) on pancreatic preparations of male Wistar-Imamichi rats in organ bath experiments was assessed by measuring insulin, amylase, and trypsin activity. Results: The level of insulin outflow remained steady in the TI-treated samples, in contrast to that in the untreated control, where insulin secretion decreased over time. The level of amylase outflow did not change significantly. Trypsin activity was significantly lower in the TI-treated samples than in the control. Conclusion: Even low concentrations of TI can maintain insulin secretion by inhibiting trypsin activity in organ bath experiments.