RT Journal Article
SR Electronic
T1 IL27 T4730C Polymorphism and Serology in Multiple Sclerosis: A Pilot Study
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 2845
OP 2853
DO 10.21873/invivo.12572
VO 35
IS 5
A1 IOANA S. BARAC
A1 VITALIE VĂCĂRAȘ
A1 ANGELA COZMA
A1 MĂDĂLINA VĂLEANU
A1 NICOLETA DECEA
A1 DAFIN F. MUREȘANU
A1 LUCIA M. PROCOPCIUC
YR 2021
UL http://iv.iiarjournals.org/content/35/5/2845.abstract
AB Background: Multiple sclerosis (MS) is one of the most debilitating neurological diseases of young adults. The presence of a single nucleotide polymorphism in the promoter regions of the interleukin 27 gene (IL27 T4730C, rs181206) may alter the transcription and the production of cytokine levels, leading to MS. Patients and Methods: We performed a case–control study including 82 individuals: 51 patients diagnosed with MS and 31 healthy controls. Polymerase chain reaction-restriction fragment length polymorphism was used in order to determine the genotypes for the IL27 T4730С polymorphism and enzyme-linked immunosorbent assay to measure the serum IL27 level. Results: Carriers of the T4730С polymorphism were found to have a 6-fold [95% confidence intervaI (CI)=1.83-19.63, p=0.002] increased risk for MS. Univariate logistic regression analysis showed an increased frequency of the TC4730 heterozygous genotype (39.2% vs. 9.7%) and also of the C4730 allele (27.45% vs. 8.06) in patients compared to controls, with a 6.02-fold increased risk (95% CI=1.61-22.46, p=0.006) and a 4.31-fold increased risk (95% CI=1.57-11.87, p=0.002) of developing MS. IL27 levels were significantly lower in patients compared to controls (12.35 versus 14.34 pg/ml, p=0.039), without significant differences between genotypes. Multivariate logistic analysis showed that IL27 T4730C polymorphism (odds ratio=6.272, 95% CI=1.84-21.40, p=0.003) and smoking (odds ratio=4.214, 95% CI=1.39-12.74, p=0.011) represented independent risk factors for MS. Conclusion: Our study provides a possible link between IL27 level and IL27 T4730C gene polymorphism and the risk for developing MS in a Romanian population.