%0 Journal Article %A MASAYOSHI OTAKE %A HIROAKI KAWAGUCHI %A SATOKO ENYA %A AKIHISA KANGAWA %A TADASHI KOGA %A KEI MATSUO %A SOHSUKE YAMADA %A MD. MAHFUZUR RAHMAN %A NAOKI MIURA %A MASATOSHI SHIBATA %A AKIHIDE TANIMOTO %T High Pathological Reproducibility of Diet-induced Atherosclerosis in Microminipigs via Cloning Technology %D 2021 %R 10.21873/invivo.12471 %J In Vivo %P 2025-2033 %V 35 %N 4 %X Background/Aim: The reproducibility of athero – sclerotic lesions was evaluated after the production of cloned-microminipigs and their offspring. Materials and Methods: Cloned-microminipig-parents were produced by microminipigsomatic cell nuclei. These parents were crossbred and delivered males (F1-offspring) were divided into two groups: normal chow diet (NcD)-fed and high-fat/high-cholesterol diet (HcD)-fed groups. One of the F1-offsprings was subjected to cloning, and delivered males (F1-clones) were fed with HcD. After 8 weeks, all animals were necropsied for patho – physiological studies compared to non-cloned-microminipigs. Results: HcD-fed F1-offspring and F1-clones, but not NcD-fed F1-offspring, exhibited increased serum lipid levels and systemic atherosclerosis, which were comparable to those of HcD-fed non-cloned-microminipigs. Homogeneity of variance analysis demonstrated that standard deviation values of serum lipoprotein and aortic atherosclerosis area from HcD-fed animals decreased in F1-offspring and F1-clones. Conclusion: HcD-induced atherogenesis was highly reproducible in F1-offsprings and F1-clones, indicating that the atherosclerosis-prone genomic background was preserved in the cloned-microminipigs, which can be used for studies on human atherosclerosis and related diseases. %U https://iv.iiarjournals.org/content/invivo/35/4/2025.full.pdf