PT  - JOURNAL ARTICLE
AU  - NATHANIEL F. WU
AU  - JUSTIN WU
AU  - JUN YAMAMOTO
AU  - YUSUKE AOKI
AU  - CHIHIRO HOZUMI
AU  - MICHAEL BOUVET
AU  - ROBERT M. HOFFMAN
TI  - The First Mouse Model of Primary Osteosarcoma of the Breast
AID  - 10.21873/invivo.12466
DP  - 2021 Jul 01
TA  - In Vivo
PG  - 1979--1983
VI  - 35
IP  - 4
4099  - http://iv.iiarjournals.org/content/35/4/1979.short
4100  - http://iv.iiarjournals.org/content/35/4/1979.full
SO  - In Vivo2021 Jul 01; 35
AB  - Background/Aim: Sarcomas of the breast are extremely rare malignant tumors and comprise only 5% of all sarcomas and fewer than 1% of breast cancers. Primary osteosarcoma of the breast is histologically indistinguishable from osteosarcoma of the bone. Effective therapies of this recalcitrant disease have not yet been developed. Materials and Methods: A patient-derived xenograft (PDX) mouse model of primary osteosarcoma of the breast was established by subcutaneous implantation of the surgical specimen, along with surrounding normal tissue. Hematoxylin and eosin (H&E) staining was performed on paraffin-embedded histological sections of the original tumor resected from the patient and from implanted tumors that grew in nude mice. Results: Tumors grew in 46 of 51 mice implanted with the original surgical specimen. The H&E-stained slides of the mouse-grown tumor and the original patient tumor matched, both showing large areas of spindle-shaped cells, characteristic of osteosarcoma. Conclusion: The first PDX mouse model of primary breast osteosarcoma was established which will enable testing of novel therapeutics as well as basic research of osteosarcoma of the breast.